5 Responses to Discovering Insect Species: the beetle DNA heads south

  1. Pingback: Discovering Insect Species: DNA magic in Arizona | The Subulate Palpomere

  2. James C. Bergdahl says:

    I am curious why there are no specimens in your survey from the western Washington region. Are Trepanedoris rare in collections from there, or it is due to a lack of specimens suitable for molecular analysis?
    James Bergdahl
    Spokane, WA, USA

    • The latter: I have done very little in the way of collecting their myself, and so I have no specimens preserved for DNA from there.

      • James C. Bergdahl says:

        Regarding Puget Sound specimens: I dug up out of my collection of pinned carabids Bembidion “acutifrons” from at least the following locales in the Puget Sound region. [I have others, especially hand collected samples from the North Cascades, between Seattle and Vancouver (BC), still in vials]. Much of the material listed below was trapped over long periods of time in 5% formalin, although at each site I typically have some specimens that I also hand-collected, especially if I had the opportunity to collect of night. All of my hand-collecting was in 75%EtOH+5% vinegar. I have no idea what impact this concentration of vinegar has on preservation of DNA; Dave Kavanaugh frequently recommended I use it to preserve morphology of genitalia.

        British Columbia, Gulf Islands: Big D’Arcy, Saturna, and Tumbo islands.
        Washington, San Juan Islands, San Juan County: San Juan, Sucia, Clark, Cabbage, Satellite, and Stuart islands.
        Washington, San Juan Islands, Skagit County: Cypress and Fidalgo islands.
        Washington, King County: numerous stillwater wetlands ca. Seattle, Issaquah, and North Bend.

        I code all of the labels of the specimens that I hand collected (“HC”), which would help identify specimens potentially suitable for molecular analysis. The specimens were collected mid-1980s through late-1990s.

        James Bergdahl
        Spokane, WA, USA

  3. Pingback: Discovering Insect Species: Overview in Rearview | The Subulate Palpomere

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