Hiding in Plain Sight

In most groups of organisms there are taxa that are very isolated phylogenetically, and are structurally so distinctive that they are easy to recognize (the Australian platypus and Welwitschia mirabilis come to mind).  Some of these isolated taxa are considered to be single species.  Within carabid beetles, some examples in North America which come to mind are Opisthius richardsoni and Gehringia olympicaIf we see a large carabid on gravel shores in western North America that has that distinctive Opisthius look (see below) we know it is of course Opisthius richardsoni.  But is it?

Opisthius richardsoni

Opisthius richardsoni

I wonder how many of these isolated, distinctive taxa actually consist of multiple species, which we haven’t discovered because we don’t bother to look closely.  Perhaps there are other species hiding in plain sight, which we could see if we only looked.

Within Bembidion, one of the more distinctive species, phylogenetically very isolated, is Bembidion (Lindrochthus) wickhami.  It is the only member of its subgenus, and is immediately recognizable because of its pretty yellow spots and its iridescent elytra (the picture, below, doesn’t do the beetle justice – it is much prettier in real life).  There is nothing else like it in North America.

Bembidion wickhami

Bembidion wickhami

Bembidion wickhami is so distinctive that I’ve never paid much attention to variation within it – if I see a spotted, small Bembidion, with rainbow iridescence on the elytra, and that distinctive prothorax, I think, “that’s a Bembidion wickhami.”  But is it?

As part of my revisionary work on North American, we are sequencing four genes (28S, COI, CAD, and topoisomerase) from five or more specimens of every species, from scattered geographic areas (if we can acquire the material).  If interesting patterns emerge in any particular species, we will sequence more if we are able.

For Bembidion wickhami, we’ve sequenced specimens from five localities: one in Oregon, three in the northern Sierra Nevada of California, and Mt Tamalpais in coastal California, just north of San Francisco (thanks Peter Hammond and Kip Will for the specimens from Mt Tam!).  We’ve sequenced a total of nine specimens, including four from Mt Tam.

wickhamiSamples

I’m sure you can guess where this is going.

Here is a portion of  28S rDNA, showing the variation in the samples.  I’ve broken the region into two pieces so that the bases will be larger and you can see them more clearly.  Remember that it is relatively rare for there to be any variation in 28S rDNA within a species of Bembidion.

wickhami28SRegion2

It might be a little hard to see, but there are three 28S forms, indicated on the left by colors:  turquoise (the Oregon specimen), pale orange (the northern Sierra specimens, plus two of the Mt Tam specimens), and dark pink (the other two Mt Tam specimens). The dark pink form is very different from the rest – six unique bases in this stretch. Here are those forms plotted on the map:

wickhamiDNA2

The COI gene shows the exact same pattern; here’s one contiguous region of that gene:

wickhamiCOI

Bottom line: there are at least two species hiding in “Bembidion wickhami“, and maybe three… or more.  I need more samples from Oregon and northern California, and until I get them I can’t be sure about the Oregon form, or whether there are even more species out there.  I also need to look for correlates between these DNA forms and the beetles’ structure.  But those four specimens from one creek shore site at Mt Tam, which have two unlinked genes (28S and COI) showing the same pattern distinguishing two groups of specimens, indicates clearly that there are two species there.

I’ve looked at the bodies of the Mt Tam specimens, and there are very slight differences between the two species in pronotal form, and color.  But they are so slight that I don’t feel confident in the consistency of the morphological differences until I get a larger sample.  There are also very slight differences in the male genitalia, but I only have one good male of the “dark pink form”, and so I can’t be sure if those differences aren’t just some individual oddity.

In a bit over a week I will fly down to visit Dave Kavanaugh, and we will head out to Mt Tam to see if we can get a large enough sample to help me really understand the species, and I will report back.  Then, more collecting in California and Oregon will be needed to help clarify the general patterns.  If any of you happen upon any “Bembidion wickhami“, and have a vial handy of 95%, I’d be delighted to sequence them…

This entry was posted in Revising Bembidiina, Taxonomic Process and tagged , , , . Bookmark the permalink.

4 Responses to Hiding in Plain Sight

  1. Jackalope says:

    Dang. That Opisthius looks like someone ran over Elaphrus with a steamroller.

  2. Pingback: Lindrochthus at Mount Tamalpais | The Subulate Palpomere

  3. Pingback: Return of the Taxa | The Subulate Palpomere

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